Background: Toxoplasmosis is a worldwide infection caused by the Toxoplasma gondii, an obligate intracellular protozoan. Approximately one-third of the world’s population is chronically infected by T. gondii. This study was conducted to determinate the anti-T. gondii antibodies (IgG and IgM) in the rural population of Qaemshahr, northern Iran in 2019. Material and methods: Sera of 350 individuals referred to the laboratories of the rural health centers in Qaemshahr city northern Iran were collected from February to April 2019 and were assessed for anti-T. gondii (IgG and IgM) antibodies using the ELISA kits (Pishtazteb, Iran). The results were analyzed using the Chi-Square test. Results: Totally, 296 (84.57%, 95% CI: 80.07-88.2 %) out of 350 sera were positive for anti-T.gondii IgG antibody. There was a significant correlation between the age group (p =0.000), soil contact (p=0.000), job and positive anti-T. gondii IgG antibody. No samples were positive for anti-T.gondii IgM antibody. No significant association was observed between the seroprevalence of T.gondii with the gender, contact with the cat and other animals, ingestion of the undercooked meat, non-chlorinated water, undercooked egg and raw milk and vegetables. Conclusions: Results of the study showed that the prevalence of toxoplasmosis was much higher among the rural individuals in the Qaemshahr city northern Iran than those previously reported from other areas of Iran. Thus, the efforts should be focused on public health education to reduce the chance of toxoplasmosis transmission especially in the high-risk groups in this region.