Background- Regulatory T lymphocytes (Tregs) play an important role in chronic liver disease progression and development of hepatocellular carcinoma. Resident Kupffer cells (KCs), the primary line of defence in the liver, are responsible for clearing the gut microbiota and maintaining liver homeostasis. Maintaining and adjusting a balance between the different immune cell populations determines the outcome of chronic liver diseases. Materials and methods- C57BL/6 mice were intraperitoneally injected with carbon tetrachloride (CCl4) twice a week for 4, 8, and 18 weeks to induce progressive liver damage. Enzymatic digestion of liver using collagenase was performed to isolate non-parenchymal cell fractions (NPF). Cell sorting using specific surface antibodies was performed to isolate KCs and Tregs from the NPF of digested livers. Results- The overall CD45+ leukocyte cell population was found to be increased in the 4 weeks CCl4 treated liver NPF in comparison to the oil-treated group, but this trend was not observed in other CCl4 treated liver NPFs. The frequency of KCs is decreased in all CCl4-treated mice liver groups in comparison to the oil-treated group. Our preliminary data also reveal that the frequency of Tregs in CD4+ cell population tends to increase after CCl4 treatment although not statistically significant when compared to the control group. However, the frequency of CD4+ was significantly decreased in all CCl4 treated liver groups in comparison to the oil treated group. Conclusion- Our preliminary results suggest a dynamic regulation of the immune cell populations analysed consequently to CCl4 induced liver damage. Our next step is to confirm these data, to fully characterize the sorted KCs and Tregs, at the epigenetic level and potentially correlate these alterations with the chronic progression of liver disease