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Cryopreservation of testicular tissues offers new possibilities to protect endangered species, genetically valuable individuals or even the fertility potential of prepubertal individuals who have died unexpectedly. However, the use of this technique still remains a challenge. In this study, slow freezing and vitrification of testicular tissue was investigated to find out which cryopreservation method could better preserve the viability and DNA integrity of testicular germ cells in diverse wild species.
This research was funded by PID2020-113288RB-100/AEI/ 10.13039/501100011033 and by the Fundación Parques Reunidos, Spain—INIA agreement CC19-096. PP-F was supported by the grant FJC2020-045827-I funded by MCIN/AEI/10.13039/501100011033 and European Union NextGeneration EU/PRTR