Impact of age and diagnosis on viability during centrifugation and cryopreservation of peripheral blood stem cell products
- Resource Type
- Authors
- Erol Ayyildiz; Meltem Bay; Pervin Topcuoglu; Osman Ilhan; S. Civriz Bozdag
- Source
- Transfusion and Apheresis Science. 47:117-120
- Subject
- Adult
Male
Pathology
medicine.medical_specialty
Cell Survival
Centrifugation
Biology
Transplantation, Autologous
Cryopreservation
Andrology
medicine
Humans
Aged
Old patients
Peripheral Blood Stem Cell Transplantation
Age Factors
Hematology
Middle Aged
Hematopoietic Stem Cells
Peripheral blood
Transplantation
Haematopoiesis
Hematological malignancy
Hematologic Neoplasms
Female
Stem cell
- Language
- ISSN
- 1473-0502
Background The viability of the hematopoietic stem cells infused to the patient is important for transplant outcome. Study design and methods We evaluated 31 peripheral blood stem cell product collected from 15 patients. We aimed to check the viabilities of the cells from patients with different age and diagnosis, in different stages of the cryopreservation procedure. Results We showed a markedly decreased viability rate after centrifugation and addition of DMSO. Percentages of viabilities were similar between young and old patients in each step. Type of hematological malignancy did not make a significant influence on the viability. Conclusion High speed centrifugation has a negative impact on the viability.