A Fluorescent Activatable AND‐Gate Chemokine CCL2 Enables In Vivo Detection of Metastasis‐Associated Macrophages
- Resource Type
- Authors
- Emily J. Thompson; Antonio Fernandez; Jeffrey W. Pollard; Takanori Kitamura; Marc Vendrell
- Source
- Fernandez, A, Thompson, E, Pollard, J W, Kitamura, T & Vendrell, M 2019, ' A Fluorescent Activatable AND-Gate Chemokine CCL2 Enables in vivo Detection of Metastasis-Associated Macrophages ', Angewandte Chemie International Edition . https://doi.org/10.1002/anie.201910955
Angewandte Chemie International Edition
Angewandte Chemie (International Ed. in English)
- Subject
- Chemokine
Lung Neoplasms
Receptors, CCR2
Apoptosis
Breast Neoplasms
CCL2
010402 general chemistry
01 natural sciences
Catalysis
Metastasis
immunology
Mice
Immune system
In vivo
fluorophores
Tumor Cells, Cultured
medicine
molecular logic
Animals
Humans
Cell Proliferation
Fluorescent Dyes
Mice, Knockout
biology
Tumour Imaging
Chemistry
010405 organic chemistry
Communication
Macrophages
imaging
General Chemistry
General Medicine
medicine.disease
Xenograft Model Antitumor Assays
Fluorescence
Communications
Molecular Imaging
Cell biology
0104 chemical sciences
3. Good health
Mice, Inbred C57BL
Spectrometry, Fluorescence
biology.protein
Female
tumour microenvironment
Chemical design
Intracellular
- Language
- ISSN
- 1521-3757
0044-8249
We report the novel chemical design of fluorescent activatable chemokines as highly specific functional probes for imaging subpopulations of immune cells in live tumours. Activatable chemokines behave as AND‐gates since they emit only after receptor binding and intracellular activation, showing enhanced selectivity over existing agents. We have applied this strategy to produce mCCL2‐MAF as the first probe for in vivo detection of metastasis‐associated macrophages in a preclinical model of lung metastasis. This strategy will accelerate the preparation of new chemokine‐based probes for imaging immune cell function in tumours.
Live show: Fluorescent activatable chemokines have been designed as the first chemical probes for in vivo detection of tumour‐associated macrophages in lung metastasis. Activatable chemokines behave as AND gates since they emit only after receptor binding and intracellular activation, thereby showing enhanced selectivity over existing agents.