Spectrophotometric study of DNA interactions with ftorafur and its elementoorganic derivatives
- Resource Type
- Authors
- Lubov Ignatovich; Calvin Yu-Chian Chen; Nikolajs Sjakste; Tatjana Sjakste; Elina Leonova; Mara Grube; Karlis Shvirksts
- Source
- Toxicological & Environmental Chemistry. 99:601-612
- Subject
- 0301 basic medicine
030102 biochemistry & molecular biology
Chemistry
Stereochemistry
Health, Toxicology and Mutagenesis
Dna interaction
Intercalation (chemistry)
Infrared spectroscopy
Antitumour drug
010402 general chemistry
01 natural sciences
Pollution
0104 chemical sciences
03 medical and health sciences
chemistry.chemical_compound
Ultraviolet visible spectroscopy
Bromide
Environmental Chemistry
DNA
DNA-ethidium
Nuclear chemistry
- Language
- ISSN
- 1029-0486
0277-2248
Ftorafur is an antimetabolite antitumour drug successfully used for treatment of various tumours. It is generally accepted that ftorafur is converted to 5-fluoruracil. However, some data indicate direct interactions of the compound with DNA. To test this hypothesis we have performed spectrophotometric study of DNA interactions of ftorafur and some of its elementoorganic derivatives with DNA. UV-VIS spectra of the tested compounds were recorded in absence and presence of increasing amounts of DNA. DNA caused a hypochromic effect in spectra of ftorafur, similar, but weaker effect was observed in 5-fluoruracil spectra. Trimethylgermyl derivative of ftorafur manifested a higher DNA-binding capacity compared to ftorafur. To reveal possible mechanism of interaction between the tested nucleosides and DNA ethidium bromide extrusion experiments were performed. It was shown that the compounds did not compete with EBr for intercalation. Fourier transformation infrared spectroscopy analyses revealed decrease ...