Additional file 1 Information of newly designed primers and GAPDH. PCR efficiency calculation with R2 value and linear dynamic range of newly designed primers. Melt curves of newly designed primers. Amplification curves of GAPDH in DF-1 cells that under went GM infection, siRNAs transfection, siRNA transfection with GM infection, or mock treatment. Melt curves of GAPDH in DF-1 cells that under went GM infection, siRNAs transfection, siRNA transfection with GM infection, or mock treatment.