Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae
- Resource Type
- Authors
- A. Prytula; D. C. T. Yong
- Source
- Journal of Clinical Microbiology. 8:643-647
- Subject
- Microbiology (medical)
chemistry.chemical_classification
Bacteriological Techniques
biology
Small volume
Microorganism
Carbohydrate
Carbohydrate metabolism
medicine.disease_cause
biology.organism_classification
Neisseria gonorrhoeae
Microbiology
Enzyme
Species Specificity
chemistry
medicine
Carbohydrate Metabolism
Humans
Neisseria
Incubation
Research Article
- Language
- ISSN
- 1098-660X
0095-1137
A rapid carbohydrate utilization procedure for the confirmation of Neisseria gonorrhoeae and identification of other Neisseria species has been developed. This method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 by 50 mm) and microamounts of media (0.05 ml) make this method not only rapid, but also low in cost.