Influence of the composition of commercial sodium dodecyl sulfate preparations on the separation of alpha- and beta-tubulin during polyacrylamide gel electrophoresis
- Resource Type
- Authors
- D. Best; P.J. Warr; K. Gull
- Source
- Subject
- Brain Chemistry
Gel electrophoresis
Sheep
Chromatography
biology
Sodium
Biophysics
Sodium Dodecyl Sulfate
chemistry.chemical_element
Cell Biology
Gel electrophoresis of proteins
Biochemistry
Electrophoresis
chemistry.chemical_compound
Tubulin
chemistry
biology.protein
Animals
Electrophoresis, Polyacrylamide Gel
Sodium dodecyl sulfate
Drug Contamination
Alkaline lysis
Molecular Biology
Polyacrylamide gel electrophoresis
- Language
- English
The α- and β-subunits of mammalian brain tubulin can be effectively separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis using the Laemmli gel system if certain commercial sodium dodecyl sulfate preparations are used in the buffers. Highly purified sodium dodecyl sulfate samples do not effect this separation. The separation is mediated by the presence of hexadecyl and tetradecyl sulfates as contaminants in certain sodium dodecyl sulfate preparations.