Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection
- Resource Type
- Authors
- S Kwok; A Friedman-Kien; Garth D. Ehrlich; Bernard J. Poiesz; John J. Sninsky; D H Mack; D Blair; Kary B. Mullis
- Source
- Europe PubMed Central
- Subject
- Immunology
Microbiology
Virus
Serology
Cell Line
Nucleic acid thermodynamics
Acquired immunodeficiency syndrome (AIDS)
Virology
medicine
Acquired Immunodeficiency Syndrome
biology
Nucleic acid sequence
HIV
Nucleic Acid Hybridization
RNA-Directed DNA Polymerase
DNA Restriction Enzymes
medicine.disease
In vitro
Oligodeoxyribonucleotides
Insect Science
DNA, Viral
biology.protein
Nucleic acid
Antibody
Research Article
- Language
- English
Human immunodeficiency virus (HIV) has been associated with acquired immunodeficiency syndrome and related disorders. Assays to detect antibodies to HIV proteins have been developed and used to screen sera for the identification of individuals who have been exposed to the virus. Although these serological tests have significant sensitivity and specificity for detecting exposure to the virus, they do not provide direct identification of HIV. We report here the application of recently developed nucleic acid amplification and oligonucleotide-based detection procedures for the identification of HIV sequences in established infected cell lines and in cells cultured from infected individuals.