Asynchronous lineage priming determines commitment to T cell and B cell lineages in fetal liver
- Resource Type
- Authors
- Ana Cumano; Rachel Golub; Paulo Vieira; Guillaume Soubigou; Pablo Pereira; Claire Berthault; Sylvestre Chea; Odile Burlen-Defranoux; Cyrille Ramond
- Source
- Nature Immunology
Nature Immunology, 2017, Epub ahead of print, ⟨10.1038/ni.3820⟩
Nature Immunology, Nature Publishing Group, 2017, ⟨10.1038/ni.3820⟩
- Subject
- 0301 basic medicine
Molecular biology
T-Lymphocytes
T cell
Immunology
Cell
Priming (immunology)
Mice, Transgenic
Biology
Immunophenotyping
Mice
03 medical and health sciences
Fetus
Precursor cell
medicine
Animals
Cluster Analysis
Immunology and Allergy
Cell Lineage
Lymphopoiesis
Progenitor cell
B cell
B-Lymphocytes
Gene Expression Profiling
Interleukin-7
Gene Expression Regulation, Developmental
Cell Differentiation
Lymphoid Progenitor Cells
Cell biology
030104 developmental biology
medicine.anatomical_structure
Liver
T cell differentiation
[SDV.IMM]Life Sciences [q-bio]/Immunology
Transcriptome
Biomarkers
Signal Transduction
- Language
- ISSN
- 1529-2916
1529-2908
International audience; The molecular events that initiate lymphoid-lineage specification remain unidentified because the stages of differentiation during which lineage commitment occurs are difficult to characterize. We isolated fetal liver progenitor cells undergoing restriction of their differentiation potential toward the T cell-innate lymphoid cell lineage or the B cell lineage. Transcripts that defined the molecular signatures of these two subsets were sequentially upregulated in lympho-myeloid precursor cells and in common lymphoid progenitor cells, respectively, and this preceded lineage restriction; this indicates that T cell-versus-B cell commitment is not a binary fate 'decision'. The T cell-bias and B cell-bias transcriptional programs were frequently co-expressed in common lymphoid progenitor cells and were segregated in subsets biased toward T cell differentiation or B cell differentiation, after interleukin 7 (IL-7) signaling that controlled the number of progenitor cells engaging in T cell differentiation versus B cell differentiation.