9 páginas.- 5 figuras.- 2 tablas.- 53 referencias.- Supplementary data associated with this article can be found in the online version at doi:10.1016/j.indcrop.2022.115658
Agro-industrial residues were used as a successful, cheap and renewable substrate, capable of increasing activity of ligninolytic enzymes for further use in melanin degradation assays to develop inputs for the treatment of skin hyperpigmentation. The analysis of melanin degradation was performed in a quantitative and qualitative way. The enzymatic crude broth extract, rich in laccases, produced by Marasmiellus palmivorus VE111 in medium formulated with lignocellulosic residues of Araucaria angustifolia subjected to hydrolysis and alcoholic fermentation, degraded almost 33% of the melanin content and enzymatic crude broth extract produced by Pycnoporus sanguineus PR_32 degraded 13%, in just 8 h, using vanillin as a mediator. Proton 2D NMR spectra show melanin changes after laccase treatment. This work has provided an insight for the development of ecofriendly, sustainable and innovative cosmetics to deal with skin hyperpigmentation. © 2022 Elsevier B.V.
The Research Support Foundation of the State of Rio Grande do Sul (Process 16/2551-0000 246-0) and the National Council for Scientific and Technological Development (CNPq) (Process 407228/2018-7 and 314897/2018-6).