Mec1 Is Activated at the Onset of Normal S Phase by Low-dNTP Pools Impeding DNA Replication
- Resource Type
- Authors
- Ismael Padioleau; Magdalena Skrzypczak; Philippe Pasero; Andrei Chabes; Antoine Barthe; Armelle Lengronne; Robin Lambert; Ana Poveda; Romain Forey; Claire Renard; Sushma Sharma; Benjamin Pardo; Krzysztof Ginalski
- Source
- Molecular Cell
Molecular Cell, Elsevier, 2020, 78 (3), pp.396-410.e4. ⟨10.1016/j.molcel.2020.02.021⟩
- Subject
- DNA Replication
Saccharomyces cerevisiae Proteins
Deoxyribonucleotides
Phase (waves)
Mitosis
Cell Cycle Proteins
Replication Origin
Saccharomyces cerevisiae
Protein Serine-Threonine Kinases
Biology
S Phase
03 medical and health sciences
0302 clinical medicine
stomatognathic system
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Gene Expression Regulation, Fungal
heterocyclic compounds
Molecular Biology
Mitotic catastrophe
030304 developmental biology
0303 health sciences
Replication timing
Replication stress
Kinase
Intracellular Signaling Peptides and Proteins
DNA replication
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Biology
Cell cycle
Budding yeast
Cell biology
enzymes and coenzymes (carbohydrates)
Checkpoint Kinase 2
biological phenomena, cell phenomena, and immunity
030217 neurology & neurosurgery
- Language
- ISSN
- 1097-2765
The Mec1 and Rad53 kinases play a central role during acute replication stress in budding yeast. They are also essential for viability in normal growth conditions, but the signal that activates the Mec1-Rad53 pathway in the absence of exogenous insults is currently unknown. Here, we show that this pathway is active at the onset of normal S phase because deoxyribonucleotide triphosphate (dNTP) levels present in G