Microarray analysis of bone marrow lesions in osteoarthritis demonstrates upregulation of genes implicated in osteochondral turnover, neurogenesis and inflammation
- Resource Type
- Authors
- Vivian Ejindu; Philip Mitchell; L. Assi; Guy Whitley; Nidhi Sofat; Ken Laing; A. Harrison; Anasuya Kuttapitiya; Caroline B. Hing; Franklyn A. Howe; Christine Heron
- Source
- Annals of the Rheumatic Diseases
- Subject
- 0301 basic medicine
Male
Pathology
Microarray
Osteoarthritis
Severity of Illness Index
Bone remodeling
0302 clinical medicine
Bone Marrow
Osteogenesis
Immunology and Allergy
Medicine
Arthroplasty, Replacement, Knee
Basic and Translational Research
Pain Measurement
Aged, 80 and over
Tissue microarray
Middle Aged
Osteoarthritis, Knee
Magnetic Resonance Imaging
Up-Regulation
medicine.anatomical_structure
Female
Bone Remodeling
Chemokines
Chondrogenesis
musculoskeletal diseases
Adult
medicine.medical_specialty
WOMAC
Neurogenesis
Immunology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Knee Osteoarthritis
Young Adult
Chondrocytes
Rheumatology
Humans
Aged
030203 arthritis & rheumatology
Inflammation
business.industry
Microarray analysis techniques
Gene Expression Profiling
medicine.disease
Gene expression profiling
030104 developmental biology
Tissue Array Analysis
Bone marrow
business
- Language
- English
- ISSN
- 1468-2060
Objective Bone marrow lesions (BMLs) are well described in osteoarthritis (OA) using MRI and are associated with pain, but little is known about their pathological characteristics and gene expression. We evaluated BMLs using novel tissue analysis tools to gain a deeper understanding of their cellular and molecular expression.\ud \ud Methods We recruited 98 participants, 72 with advanced OA requiring total knee replacement (TKR), 12 with mild OA and 14 non-OA controls. Participants were assessed for pain (using Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC)) and with a knee MRI (using MOAKS). Tissue was then harvested at TKR for BML analysis using histology and tissue microarray.\ud \ud Results The mean (SD) WOMAC pain scores were significantly increased in advanced OA 59.4 (21.3) and mild OA 30.9 (20.3) compared with controls 0.5 (1.28) (p