Individuals with Type I Diabetes Mellitus (T1DM) are at an increased risk for developing secondary complications which increases their risk of premature death. While the risk of secondary complications is reduced with intensive insulin treatment (IIT) and aerobic exercise (AE), both interventional strategies increase the risk of hypoglycemia. This study examined the effects of a 60-min bout of forced treadmill AE on hepatic and muscular blood glucose (BG) metabolism. Nineteen Sprague-Dawley rats were divided into two groups: Sedentary Control Rats (SC; n=5), and Sedentary T1DM rats (DSC); T1DM rats were subcategorized into Diabetic Pre-Exercise (DPRE; n=14) and Diabetic Post-Exercise (DPOST; n=7). The study was conducted over twelve weeks and upon completion the DPOST group underwent a 60-min bout of AE. Immediately following the experimental protocol both liver and muscle tissue were analyzed to measure BG, hepatic and muscular glycogen, and several liver and muscular regulatory enzymes (G6Pase, PEPCK, AKT). The DPOST rats had significantly lower hepatic glycogen when compared to the SC group but there was no difference when compared to the DPRE rats. G6Pase protein content was greater in the DPOST when compared to the SC group. Phosphorylated AKT (P-AKT) was greater in DPOST rats when compared with both SC and DPRE groups. However, no significant differences were found in PEPCK protein content in response to diabetes and exercise. These findings suggest that during AE in T1DM rats both gluconeogenesis and both hepatic and muscle glycogen is underutilized leading to a reliance on circulating BG to meet the increased metabolic demands during exercise.