PDF file - 542K, A. MCF-7/MUC1-C cells were treated with 10 μM LY294002 (LY) for the indicated times. Lysates were immunoblotted with the indicated antibodies. B. MCF-7/MUC1-C cells were left untreated (Control) or treated with 10 μM LY294002 for 48 h. Aliquots of control and LY294002-treated cells were then incubated in the presence of 5 μM tamoxifen for an additional 48 h. Cell viability at 96 h was determined by trypan blue staining. The results (mean+SD of three determinations) are expressed as percentage cell death.