Similar kinetic characteristics of 5-hydroxytryptamine binding in blood platelets and brain membranes of rats
- Resource Type
- Authors
- C.G. Honegger; A. Pletscher; H.P. Von Hahn
- Source
- Neuroscience Letters. 20:319-322
- Subject
- Blood Platelets
Brain Chemistry
Serotonin
Binding Sites
Membranes
Chemistry
General Neuroscience
Brain
Rats
Kinetics
Membrane
5-hydroxytryptamine binding
Rats, Inbred Lew
Receptors, Serotonin
Immunology
Lewis rats
Biophysics
Animals
Female
Platelet
Binding site
Receptor
- Language
- ISSN
- 0304-3940
Blood platelets and brain membranes of SIV and Lewis rats both exhibited two saturable binding sites for 5-hydroxytryptamine (5-HT) in the concentration range 1-100 nM. The Kd values for the high-affinity sites were 4-6 nM and for the low-affinity sites 20-40 nM in both tissues of both rat strains. Blood platelets had 100-200 times more binding sites per mg protein (Bmax) than brain membranes. Thus, 5-HT receptors of platelets may be used as models for those of cerebral 5-HT-neurons.