DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer
- Resource Type
- Authors
- Atsushi Arakaki; Brandon Yoza; Tadashi Matsunaga
- Source
- Journal of biotechnology. 101(3)
- Subject
- Dendrimers
Macromolecular Substances
Polymers
Iron
Bioengineering
Applied Microbiology and Biotechnology
chemistry.chemical_compound
Magnetics
Coated Materials, Biocompatible
Suspensions
Dendrimer
Polymer chemistry
Polyamines
Humans
Particle Size
Magnetite
Aqueous solution
Microchemistry
Extraction (chemistry)
Oxides
General Medicine
DNA
Silane
Ferrosoferric Oxide
Microspheres
Rhodospirillaceae
chemistry
Chemical engineering
Magnetic nanoparticles
Particle size
Dispersion (chemistry)
Biotechnology
- Language
- ISSN
- 0168-1656
A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane.