Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches
- Resource Type
- Authors
- Pravin R. Kaldhone; Steven L. Foley; Bijay K Khajanchi
- Source
- Microbial Transposon Mutagenesis ISBN: 9781493995691
- Subject
- Genetics
Transposable element
0303 health sciences
Salmonella
biology
030306 microbiology
Bacterial conjugation
medicine.disease_cause
biology.organism_classification
03 medical and health sciences
chemistry.chemical_compound
Plasmid
chemistry
medicine
Gene
DNA
Horizontal transmission
Bacteria
030304 developmental biology
- Language
Bacterial conjugation is a natural process that allows for horizontal transmission of DNA from one bacterium to another. Several plasmids carry transposons that encode multiple antimicrobial and metal resistance genes. Conjugative plasmid transfer requires intimate cell-to-cell contacts between the donor and the recipient. Self-conjugative plasmids harbor tra genes which facilitate plasmid transfer from donor to recipient bacterial strain. Here we describe different methods of conjugative plasmid transfers via conjugation.