PDF file - 6761K, Correlation of HER-2/neu with pCREB expression. (A) shHER-2 transfection strongly down-regulates HER-2/neu expression in MCF-7 cells. (B) shHER-2/neu-mediated down-regulation of HER-2/neu in HER-2/neu+ MCF-7 cells results in a reduced in CREB phophorylation. (C) Representative immunohistochemical staining and grading from breast cancer tumor lesions are shown.