Pulmonary Tuberculosis among Suspected Sudanese Patients in Wad Madani Tuberculosis Center
- Resource Type
- Authors
- Abdelrhman E. Mohammed; Zeinab Hamid Elfaham; Elhadi A. Ahmed; Bakri Y. M. Nour; Elamin M. Ibrahim
- Source
- Open Journal of Medical Microbiology. 11:298-307
- Subject
- medicine.medical_specialty
GeneXpert MTB/RIF
Tuberculosis
biology
business.industry
General Medicine
medicine.disease
biology.organism_classification
Stain
Mycobacterium tuberculosis
Pulmonary tuberculosis
Internal medicine
Infection control
Medicine
Sputum
medicine.symptom
business
- Language
- ISSN
- 2165-3380
2165-3372
Background: Tuberculosis is a health problem in Sudan and may become a greater challenge in the future due to the weakness in infection prevention measures, increase in cases of drug-resistant and the difficulty of diagnosis. Objective: The aim of this study was to detect Mycobacterium tuberculosis (MTB) from sputum of clinically suspected patients using the three available techniques. Methods: Three hundred participants referred to Wad Madani Tuberculosis Center during 2017-2019 were included. Early morning fresh sputum samples were subjected to Mycobacterium tuberculosis examination by Ziehl-Neelsen (ZN) stain without concentration, ZN stain with centrifugation and geneXpert assay. Results: Of the 300 suspected cases; Mycobacterium tuberculosis detected in 17% (51/300) by ZN stain without concentration, 20% (59/300) by ZN stain with centrifugation and 34% (103/300) by geneXpert. The two techniques of ZN stains possessed 100% specificity and relative differences in sensitivity when compared to geneXpert assay. The significant association observed between ZN stains and geneXpert results indicated validity of ZN techniques for detection. Conclusions: The study confirmed that geneXpert is better for identification of Mycobacterium tuberculosis when compared to ZN techniques which are also important for diagnosis in poor places and where the geneXpert assay is not available.