A Checkpoint-oriented cell cycle simulation model
- Resource Type
- Authors
- Sylvain Cussat-Blanc; David Bernard; Aurélie Gomes; Odile Mondesert; Valérie Lobjois; Yves Duthen; Bernard Ducommun
- Source
- Cell Cycle
Cell Cycle, Taylor & Francis, 2019, 18 (8), pp.795-808. ⟨10.1080/15384101.2019.1591125⟩
Cell Cycle, 2019, 18 (8), pp.795-808. ⟨10.1080/15384101.2019.1591125⟩
- Subject
- 0301 basic medicine
In silico
Population
Mitosis
Computational biology
Biology
[INFO] Computer Science [cs]
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Exponential growth
Tumor Microenvironment
Humans
Computer Simulation
[INFO]Computer Science [cs]
education
Molecular Biology
Cell Proliferation
Confluency
education.field_of_study
Nocodazole
agent-based modeling
Cell Biology
Cell cycle
HCT116 Cells
G1 Phase Cell Cycle Checkpoints
Kinetics
030104 developmental biology
Eukaryotic Cells
in silico simulation
chemistry
030220 oncology & carcinogenesis
Cancer cell
Colonic Neoplasms
cell cycle
synchronization
Developmental Biology
Research Paper
- Language
- English
- ISSN
- 1538-4101
1551-4005
International audience; Modeling and in silico simulations are of major conceptual and applicative interest for studying the cell cycle and proliferation in eukaryotic cells. In this paper, we present a cell cycle checkpoint-oriented simulator that uses agent-based simulation modeling to reproduce the dynamics of a cancer cell population in exponential growth. Our in silico simulations were successfully validated by experimental in vitro supporting data obtained with HCT116 colon cancer cells. We demonstrated that this model can simulate cell confluence and the associated elongation of the G1 phase. Using nocodazole to synchronize cancer cells at mitosis, we confirmed the model predictivity, and provided evidence of an additional and unexpected effect of nocodazole on the overall cell cycle progression. We anticipate that this cell cycle simulator will be a potential source of new insights and research perspectives.