The bio-chemically selective interaction of hydrogenated and oxidized ultra-small nanodiamonds with proteins and cells
- Resource Type
- Authors
- Martin Hubálek; Tereza Belinova; Anna Fucikova; Stepan Stehlik; Bohuslav Rezek; Marie Hubalek Kalbacova; Iva Machova
- Source
- Carbon. 162:650-661
- Subject
- chemistry.chemical_classification
Biomolecule
Nanoparticle
Protein Corona
02 engineering and technology
General Chemistry
010402 general chemistry
021001 nanoscience & nanotechnology
Mass spectrometry
01 natural sciences
0104 chemical sciences
Protein–protein interaction
Cell membrane
medicine.anatomical_structure
chemistry
medicine
Biophysics
General Materials Science
0210 nano-technology
Cytotoxicity
Quantitative analysis (chemistry)
- Language
- ISSN
- 0008-6223
Ultra-small nanoparticles of a size smaller than or comparable to cell membrane pores (1–5 nm) offer significant potential in the field of biomedicine. This study presents a systematic in vitro investigation of fundamental bio-chemical interactions of such ultra-small hydrogenated and oxidized detonation nanodiamonds (DNDs) with biomolecules and human cells. We apply mass spectrometry methods (LC-MS/MS) for the qualitative and quantitative analysis of the protein corona as a function of the surface chemistry and size of DNDs. We observe that protein interactions with DNDs are more related to their surface chemistry (H/O-termination) rather than size. Bioinformatics characterization of the identified proteins points to the strong influence of electrostatic interaction between proteins and DNDs depending on their termination. Such specific interaction leads to formation of different protein corona on 2 nm DNDs, which influences also interaction with cells including different level of cytotoxicity.