A novel fluorescent biosensor for detection of Pb2+based on Pb2+-specific DNAzyme and carbon dots prepared from watermelon rind
- Resource Type
- Article
- Authors
- Zhao, Weiqin; Huang, Chun; Li, Yuangang; Wei, Bizhuo; Gao, Jie
- Source
- Chemistry Letters; January 2024, Vol. 53 Issue: 1
- Subject
- Language
- ISSN
- 03667022; 13480715
A novel fluorescent biosensor was constructed to detect Pb2+. Multifunctional magnetic beads modified with substrate sequence and Pb2+-specific DNAzyme were employed as recognition probes. The fluorescence of carbon dots synthesized from watermelon rind (W-CDs) was quenched by hemin. Hemin/W-CDs were employed as fluorescent signals. The presence of Pb2+could target-trigger the biosensor, and generated G-quadruplex which could restore the fluorescence of hemin/W-CDs. The fluorescence change of the biosensor depended on Pb2+concentration from 1 to 20 nM.A fluorescent biosensor was constructed to detect Pb2+. Magnetic beads modified with substrate sequence and Pb2+-specific DNAzyme were employed as recognition probes. The fluorescence of carbon dots synthesized from watermelon rind (W-CDs) was quenched by hemin. Hemin/W-CDs were employed as fluorescent signals. The presence of Pb2+could target-trigger the biosensor, and generated G-quadruplex which could restore the fluorescence of hemin/W-CDs.