Lipoxygenase (LOXs) is a kind of dioxygenase without heme and iron, which plays an important role in the development and adaptation of many plants to the environment. However, the study of strawberry LOX gene family has not been reported. In this study, 14 LOX genes were identified from the diploid woodland strawberry genome. The phylogenetic tree divides the FvLOXgene into two subfamilies: 9-LOX and 13-LOX. Gene duplication event analysis showed that whole-genome duplication (WGD)/segmental duplication and dispersed duplication effectively promoted the expansion of strawberry LOX family. QRT-PCR analysis showed that FvLOXgenes were expressed in different tissues. Expression profile analysis showed that FvLOX1and FvLOX8were up-regulated under low temperature stress, FvLOX3and FvLOX7were up-regulated under drought stress, FvLOX6and FvLOX9were up-regulated under salt stress, FvLOX2, FvLOX3and FvLOX6were up-regulated under salicylic acid (SA) treatment, FvLOX3, FvLOX11and FvLOX14were up-regulated under methyl jasmonate (MeJA) treatment, FvLOX4and FvLOX14were up-regulated under abscisic acid (ABA) treatment. Promoter analysis showed that FvLOXgenes were involved in plant growth and development and stress response. We analyzed and identified the whole genome of strawberry FvLOXfamily and characterized a variety of FvLOXcandidate genes involved in abiotic stress response. This study laid a theoretical and empirical foundation for the response mechanism of strawberry to abiotic stress.