Arcobacteris considered an emergent foodborne enteropathogen. Despite the high prevalence of this genus in poultry, the occurrence of Arcobacterspp. contamination in Tunisia remains unclear. The objectives of this study were (i) to isolate Arcobacterspecies (A. butzleriand A. cryaerophilus) by the culture method from different species of raw poultry meat, (ii) to verify the isolates by multiplex PCR (m-PCR) assay and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS), and (iii) to determine the antibiotic resistance profiles of the isolates. A total of 250 poultry product samples (149 chicken and 101 turkey) were collected from various supermarkets in Sfax. The samples consisted of breasts, wings, legs, and neck skins. The overall isolation frequency of Arcobacterspp. was 10.4%. Arcobacterspp. were found in 13.42% of the chicken samples and in 5.49% of the turkey samples. All the acquired isolates were subject to detailed confirmation with subsequent species classification using m-PCR and MALDI-TOF MS. A. butzleriwas found in 22 samples (84.61%) and A. cryaerophilusin 4 samples (15.38%). Thus, m-PCR and MALDI-TOF MS were able to detect A. butzlerisignificantly better than the conventional method (χ2= 49.1 and P< 0.001). Arcobacterwas isolated from poultry in every season, at contamination levels of 30.76, 23.07, 19.23, and 26.92% in summer, spring, autumn, and winter, respectively. The disk diffusion method was used to determine the susceptibility of Arcobacterisolates to six antimicrobial drugs. All A. butzleriisolates (n= 24) were significantly resistant to erythromycin (P= 0.0015), ampicillin (P= 0.001), and ciprofloxacin (P= 0.05). All tested A. cryaerophilusstrains (n= 4) were susceptible to ampicillin, gentamicin, and amoxicillin–clavulanic acid. Multidrug resistance was observed in 83% of the Arcobacterspp. isolates. Our study detected Arcobacterspp. in Tunisian poultry; because of their multidrug resistance, these species may constitute a public health problem.