Graphical Abstract: This work proposes a bioactive fiber-based sensor patch comprising a non-enzyme Co3O4/carbon fiber-based biorecognition element and a temperature calibration unit. The sole fiber-based sensor could selectively monitor dopamine and lactic acid contents based on cyclic voltammetry and amperometry, exhibiting a wide detection range, excellent selectivity and reproducibility with temperature calibration in an ambient temperature range from 25 to 45 ℃, promising personalized and preventative health care in various scenarios