Mutations in the presenilin 1(PS1) and presenilin 2(PS2) genes cause the most common and aggressive form of early onset familial Alzheimer's disease. To elucidate their pathogenic mechanism, wild-type (wt) or mutant (M146L, C410Y) PS1and wt or mutant (M239V) PS2genes were stably transfected into Chinese hamster ovary cells that overexpress the β-amyloid precursor protein (APP). The identity of the 43-45-kDa PS1 holoproteins was confirmed by N-terminal radiosequencing. PS1 was rapidly processed (t1/2= 40 min) in the endoplasmic reticulum into stable fragments. Wild-type and mutant PS2 holoproteins exhibited similar half lives (1.5 h); however, their endoproteolytic fragments showed both mutation-specific and cell type-specific differences. Mutant PS1 or PS2 consistently induced a 1.4-2.5-fold increase (p< 0.001) in the relative production of the highly amyloidogenic 42-residue form of amyloid β-protein (Aβ42) as determined by quantitative immunoprecipitation and by enzyme-linked immunosorbent assay. In mutant PS1 and PS2 cell lines with high increases in Aβ42/Aβtotalratios, spontaneous formation of low molecular weight oligomers of Aβ42was observed in media, suggesting enhanced Aβ aggregation from the elevation of Aβ42. We conclude that mutant PS1 and PS2 proteins enhance the proteolysis of β-amyloid precursor protein by the γ-secretase cleaving at Aβ residue 42, thereby promoting amyloidogenesis.