Cell Line-dependent Response of the Enhancer element of Simian Virus 40 to Transactivator p40taxEncoded by Human T-cell Leukemia Virus type I*
- Resource Type
- Article
- Authors
- Nakamura, M; Niki, M; Nagata, K; Ohtani, K; Saito, S; Hinuma, Y; Sugamura, K
- Source
- Journal of Biological Chemistry; December 1989, Vol. 264 Issue: 34 p20189-20192, 4p
- Subject
- Language
- ISSN
- 00219258; 1083351X
The trans-actingtranscriptional factor p40taxof human T-cell leukemia virus type I (HTLV-I) can activate expression not only of its own viral genes but also of other viral and cellular genes. We examined the cis-acting sequences in the simian virus 40 (SV40) enhancer required for its activation by HTLV-I p40tax. Experiments with chloramphenicol acetyltransferase constructs bearing the SV40 enhancer elements revealed that p40tax-dependent transactivation of the SV40 enhancer is mediated through the C element that contains the typical sequence for binding of a nuclear factor NF-κB. Activation of the C element by p40taxwas seen in a limited set of cell lines as was also seen with the whole enhancer of SV40. Binding of NF-κB or NF-κB-like factor to the SV40 C element increased when p40taxwas expressed. The fact that HTLV-I p40taxutilizes a cellular regulatory mechanism to transactivate the SV40 enhancer in a cell-dependent manner may have implications for the pathogenesis of adult T-cell leukemia.