NAC proteins in plants respond to stress and play an important role in plant growth regulation. This regulation occurs through a variety of downstream effects mediated by plant hormones in response to stress. In this study, we performed a systematic genome-wide analysis of the NACgene family in tomato based on genome version SL4.0. We identified 99 SlNACgenes by abiotic stress analysis of conserved motifs and gene structure, phylogenetic analysis, cis-element analysis, chromosome localization analysis, synteny relationship and expression pattern analysis. These 99 SlNACgenes were distributed on all 12 chromosomes. Except for genes without introns, there was no significant correlation between gene structures and phylogenetic relationships. Most of the cis-elements identified were associated with plant hormones and environmental responses. There were 77 SlNACgenes that showed no homogeneity between tomato and wild rice, suggesting that these homologous genes arose after the differentiation of dicotyledonous and monocotyledonous plants. The comprehensive analysis of the SlNACgene family provides a valuable resource for studying this gene family in tomato and a foundation for further study of the functional characteristics of these genes.