Presence of free viral DNA in simian virus 40-transformed nonproducer cells
- Resource Type
- Article
- Authors
- Daya-Grosjean, L; Monier, R
- Source
- The Journal of Virology; August 1978, Vol. 27 Issue: 2 p307-312, 6p
- Subject
- Language
- ISSN
- 0022538X; 10985514
Extracts from several simian virus 40 (SV40)-transformed nonproducer cells were prepared by the hot-phenol procedure normally used to extract cellular RNA. These extracts contained SV40 infectious units. Part of the infectious units were identified as SV40 form I DNA molecules. The results of reconstruction experiments suggest that SV40 form I DNA is extractable by the hot-phenol procedure because of its fast renaturation rate. The significance of the presence of free viral DNA in nonproducer transformed cells is discussed.