In this study, the degradation of the subthreshold swing (S.S.) and the hump effect are observed in the local oxidation of silicon (LOCOS) metal-oxide-semiconductor field-effect transistors (MOSFETs) under short- and long-term positive bias temperature instability tests, respectively. S.S. collapse is considered to be caused by anode hot-hole injection, and the hump effect occurs because the geometric structure from LOCOS isolation forms parasitic transistors on both sides of the channel, which can be verified by a Silvaco Technology computer-aided design (TCAD) simulation. In addition, the body effect measurement method is proposed to elucidate the mechanism of the hump effect for further confirmation of hump formation.