The nucleotide sequences of five major regions from chromosome VII of Saccharomyces cerevisiaehave been determined and analysed. These regions represent 203 kilobases corresponding to approximately one‐fifth of the complete yeast chromosome VII. Two fragments originate from the left arm of this chromosome. The first one of about 15·8 kb starts approximately 75 kb from the left telomere and is bordered by the SKI8chromosomal marker. The second fragment covers the 72·6 kb region between the chromosomal markers CYH2and ALG2. On the right chromosomal arm three regions, a 70·6 kb region between the MSB2and the KSS1chromosomal markers and two smaller regions dominated by the KRE11marker and another one in the vicinity of the SER2marker were sequenced.