Anaplasmataceae family members include vector-borne bacteria of veterinary importance that may also affect humans. Ehrlichia canisand Anaplasma platysare the main members of this family detected in dogs worldwide. In Uruguay there are not many published studies on tick-borne pathogens affecting dogs, the only haemoparasite molecularly confirmed in dogs, is the piroplasm Rangelia vitalii. The aim of the present work was to detect the presence of A. platysand E. canisin dogs and dogs-associated ticks of two localities in Northwestern Uruguay. Blood samples from dogs with and without clinical signs associated with vector-borne diseases, and Rhipicephalus sanguineusobtained from these dogs were analyzed by PCR for Anaplasmataceae. Positive dogs were further analyzed by PCR for Ehrlichiaspp. and A. platys. All the ticks were found negative. No dog was detected infected with E. canis,while eight dogs (4.2%) were found to be infected with A. platys. Phylogenetic analysis of groESLoperon sequence for A. platysrevealed no differences with sequences described for A. platysin neighbor countries and from other regions of the world. This is the first report of the presence of A. platysin Uruguay.