In vitro produced embryos have lower cryotolerance than the in vivo counterparts, resulting in lower pregnancy rates when they are cryopreserved and transferred; thus, transporting fresh embryos (not frozen) is a viable alternative to obtain good pregnancy outcomes. The aim of this work was to evaluate different systems for transportation of fresh in vivo- and in vitro- produced embryos up to 8h. In vivo produced embryos were collected nonsurgically from superovulated cows. Also, oocytes were collected from slaughterhouse ovaries, matured, fertilized, and the resulting embryos cultured in vitro by standard procedures in a chemically defined medium. Day 0 of culture was ~18 h after the onset of in vitro fertilization. Seven days after fertilization or insemination, respectively, in vitro- and in vivo-produced embryos were blocked by quality and stage and allocated to different treatments in a 2 x 2 x 2 factorial design, with factors: embryo origin (in vivo (n=32) vs. in vitro (n=69)), media (mPBS with 0.05% PVA vs. HCDM2 with 0.4% BSA), and temperature (25 vs. 37°C). The embryos were blindly and subjectively evaluated for stage (5=early blastocyst, 6=blastocyst, 7=expanded blastocyst and 8=hatched blastocyst) and quality (1=excellent, 2=good, 3=fair and 4=dead) by a single person every 2 h during an 8 h period (Time). An ANCOVA model to adjust storage time as a covariate was used; main effects and first, second, and third order interactions of fixed factors were included in the model. Second order interactions were not significant and dropped from the model. Two way significant interactions for embryo quality were: temperature X embryo, media X time and temperature X time (P < 0.05). In vitro-produced embryos were better kept at 25°C (quality 1.56 vs. 2.00) whereas in vivo produced embryos quality was best at 38°C (quality 2.02 vs. 1.75). HCDM2 medium resulted in better quality embryos with time. Quality of embryos with time was better under 25°C. There were no embryo X media or time interactions (P > 0.1). For stage, significant interactions were temperature X embryo and media X time (P < 0.05). In vivo produced embryos progressed better in stage at 25°C whereas in vitro embryos performed better at 38°C. Embryos placed in mPBS progressed faster than in HCDM2. In summary, HCDM2 was better for long time embryo transportation, 25°C was best for in vitro produced embryos, whereas for in vivo produced embryos 38°C was best. For short time of transportation (2 h) both temperatures are appropriate. However, 25°C is better suited for long time transportation (8 h). Research supported by Fondos Mixtos del Estado de Chihuahua-CONACYT, Grant No. CHIH-2008-C01-91923.(poster)