Efficient Transduction of Alveolar Type 2 Cells with Adeno-associated Virus for the Study of Lung Regeneration.
- Resource Type
- Article
- Authors
- Rindler, Tara N.; Brown, Kari M.; Stockman, Courtney A.; van Lieshout, Laura P.; Martin, Emily P.; Weaver, Timothy E.; Zacharias, William J.; Wootton, Sarah K.; Whitsett, Jeffrey A.; Bridges, James P.
- Source
- American Journal of Respiratory Cell & Molecular Biology; Jul2021, Vol. 65 Issue 1, p118-121, 4p
- Subject
- ANGIOTENSIN II
CELL physiology
TRANSGENIC mice
INTERSTITIAL lung diseases
RECOMBINASES
- Language
- ISSN
- 10441549
The article presents a study of monogenetic disorders affecting Angiotensin II (AT2) cell function and causing Interstitial Lung Disease (ILD) is limited by the time and expense needed to produce transgenic mice to delete or mutate genes of interest. It mentions production of AAV6.2FF-Cre to express Cre-recombinase in AT2 cells to eliminate the need for biallelic transgenic mice.