DNA Damage in Peripheral Blood Leukocytes of Physically Active Individuals as Measured by the Alkaline Single Cell Gel Electrophoresis Assay.
- Resource Type
- Article
- Authors
- Gandhi, Gursatej; Chopra, Gunjan
- Source
- Environmental & Molecular Mutagenesis; May2009, Vol. 50 Issue 4, p291-303, 13p
- Subject
- DNA damage
LEUCOCYTES
GEL electrophoresis
PHYSICAL fitness
BLOOD testing
EXERCISE physiology
PHYSICAL education
PHYSICAL fitness testing
- Language
- ISSN
- 08936692
The article presents a study which investigates the DNA damage in peripheral blood leukocytes of physically active individuals using the alkaline single cell gel electrophoresis (SCGE) assay. The subject group in the study was identified through a survey and were questioned regarding their fitness schedules and personal histories. Blood samples were collected from the individuals 15 minutes after the maximal oxygen uptake (VO2) but before exercising, and were processed for the SCGE assay. Results show an increased percentage of cells and increased in mean DNA migration length in blood leukocytes of the subject group. SCGE shows the induction of DNA damage in human leukocytes independent of the exercise type performed.