Low molecular weight peptide from calf's liver mitochondrial DNA: structure and effect on DNA as a template.
- Resource Type
- Academic Journal
- Authors
- Spena A; Department of Cell Biology, University of Calabria, Rende (CS), Italy.; Chimenti R; Covello C; De Cicco T; Mazzulla S; Martino G
- Source
- Publisher: Pacific Press Country of Publication: United States NLM ID: 8502230 Publication Model: Print Cited Medium: Print ISSN: 0748-6642 (Print) Linking ISSN: 07486642 NLM ISO Abbreviation: Physiol Chem Phys Med NMR Subsets: MEDLINE
- Subject
- Language
- English
- ISSN
- 0748-6642
A peptide fraction from the mitochondrial DNA of calf's liver was isolated using Drouin's method (1). This peptide fraction, which was extracted at pH 9.5 from an extensively purified mitochondrial DNA (2), has been shown to exert an in vitro regulatory role on the transcription and duplication activity of DNA (3). The same fraction also binds with mitochondrial DNA with a high affinity constant and stabilizes DNA from calf's thymus against thermal denaturation. The peptides from mitochondrial DNA have been subfractionated by fingerprinting-like techniques and one of them has been sequenced.