Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer.
- Resource Type
- Academic Journal
- Authors
- Wang ZD; Department of Thoracic Surgery, Shandong University Cancer Center, Jinan, China.; Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.; Feng YF; School of Clinical and Basic Medicine, Shandong First Medical University, Jinan, China.; Wang YS; Department of Primary Care and Population Health, Institute of Epidemiology and Public Health, London's Global University, London, UK.; Ma Y; Shandong Pharmaceutical Research Institute, Jinan, China.; Liu J; Shandong Pharmaceutical Research Institute, Jinan, China.; Li D; Shandong Pharmaceutical Research Institute, Jinan, China.; Li S; Shandong Pharmaceutical Research Institute, Jinan, China.; Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.; Zhang GD; Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.
- Source
- Publisher: Wiley Publishing Asia Pty Ltd Country of Publication: Singapore NLM ID: 101531441 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1759-7714 (Electronic) Linking ISSN: 17597706 NLM ISO Abbreviation: Thorac Cancer Subsets: MEDLINE
- Subject
- Language
- English
Background: Circulating tumor cells (CTCs) play a crucial role in the early diagnosis and prognosis of lung cancer. Identification of a more suitable sample source could be a breakthrough towards enhancing CTC detectability in early-stage lung cancer. We investigated the differences in detectable CTCs between peripheral arterial and venous blood in early- and mid-stage lung cancer patients undergoing surgery and analyzed the association between clinicopathological factors and detectable CTCs in peripheral arterial and venous blood.
Methods: Peripheral arterial and venous blood was collected in 5-mL samples from 56 patients with surgically resected and pathologically clear at early- or mid-stage lung cancer. Blood specimens were enriched for CTCs based on isolation by size of epithelial tumor cells. The CTCs were identified using Swiss Giemsa staining and immunohistochemistry for CD45/CD31.
Results: In stage I lung cancer, CTC-positive rate was significantly higher in peripheral arterial than in venous blood (45.45% vs. 17.39%). There was no significant difference in the number of detectable CTCs between peripheral arterial and venous blood. A low degree of differentiation was associated with a high positive rate of CTCs in peripheral venous blood. The number of circulating tumor microemboli was significantly higher in patients with tumor size >3 cm compared with ≤3 cm.
Conclusion: CTC levels in peripheral arterial and venous blood differed little in lung cancer patients.Compared to peripheral venous blood, peripheral arterial blood had a higher CTC positivity rate in early-stage lung cancer.This study was favorable for early detection and monitoring of lung cancer.
(© 2024 The Authors. Thoracic Cancer published by John Wiley & Sons Australia, Ltd.)