Single Chain Cationic Polymer Dot as a Fluorescent Probe for Cell Imaging and Selective Determination of Hepatocellular Carcinoma Cells.
- Resource Type
- Academic Journal
- Authors
- Özenler S; Department of Chemistry , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Yucel M; Department of Bioengineering , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Tüncel Ö; Department of Chemistry , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Kaya H; Department of Bioengineering , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Özçelik S; Department of Chemistry , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Department of Photonics , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Department of Radiation Oncology, School of Medicine , Stanford University , Palo Alto , California 94305 , United States.; Yildiz UH; Department of Chemistry , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.; Department of Photonics , Izmir Institute of Technology , Urla 35430 , Izmir , Turkey.
- Source
- Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE
- Subject
- Language
- English
This letter describes formation of single chain cationic polymer dots (Pdots) made of poly[1,4-dimethyl-1-(3-((2,4,5-trimethylthiophen-3-yl)oxy)propyl)piperazin-1-ium bromide] conjugated polyelectrolyte (CPE). The single chain Pdot formation relies on a simple process which is a rapid nanophase separation between CPE solution of ethylene glycol and water. Pdots show narrow monodisperse size distribution with a 3.6 nm in diameter exhibiting high brightness and excellent colloidal and optical stability. It has been demonstrated that photoluminescent Pdots provide selective nuclear translocation to hepatocellular carcinoma cells as compared to healthy liver cells. The Pdot labeling effectively discriminates cancer cells in the coculture media. Pdots hold great promise as a luminescent probe to diagnose cancer cells in histology and may guide surgeons during operations to precisely separate out cancerous tissue due to augmented fluorescence brightness.