Proteomic identification of metabolic changes in Paracoccidioides brasiliensis induced by a nitroheteroarylchalcone.
- Resource Type
- Academic Journal
- Authors
- Júnior MA; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Silva LC; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Rocha OB; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Oliveira AA; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Portis IG; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Alonso A; Institute of Physics, Federal University of Goiás, Goiânia, Goiás, Brazil.; Alonso L; Institute of Physics, Federal University of Goiás, Goiânia, Goiás, Brazil.; Silva KS; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Gomes MN; InsiChem, Goiás State University, Anápolis, Goiás, Brazil.; Faculdade Metropolitana de Anápolis, Anápolis, Goiás, Brazil.; Andrade CH; Laboratory for Molecular Modeling & Drug Design, Faculty of Pharmacy, Federal University of Goiás, Goiânia, Goiás, Brazil.; Soares CM; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.; Pereira M; Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Goiás, Brazil.
- Source
- Publisher: Future Medicine Country of Publication: England NLM ID: 101278120 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1746-0921 (Electronic) Linking ISSN: 17460913 NLM ISO Abbreviation: Future Microbiol Subsets: MEDLINE
- Subject
- Language
- English
Aim: To access the metabolic changes caused by a chalcone derivative ( LabMol-75 ) through a proteomic approach. Methods: Proteomic analysis was performed after 9 h of Paracoccidioides brasiliensis yeast ( Pb18 ) cell incubation with the LabMol-75 at MIC. The proteomic findings were validated through in vitro and in silico assays. Results: Exposure to the compound led to the downregulation of proteins associated with glycolysis and gluconeogenesis, β-oxidation, the citrate cycle and the electron transport chain. Conclusion: LabMol-75 caused an energetic imbalance in the fungus metabolism and deep oxidative stress. Additionally, the in silico molecular docking approach pointed to this molecule as a putative competitive inhibitor of DHPS.