Biocompatibility of biomimetic multilayered alginate-chitosan/β-TCP scaffold for osteochondral tissue.
- Resource Type
- Academic Journal
- Authors
- Algul D; Department of Pharmaceutical Technology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul 34755, Turkey; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul 34116, Turkey. Electronic address: deryaalgul@gmail.com.; Sipahi H; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul 34755, Turkey.; Aydin A; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul 34755, Turkey.; Kelleci F; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Yeditepe University, Kayisdagi, Istanbul 34755, Turkey.; Ozdatli S; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Medipol University, Beykoz, Istanbul 34810, Turkey.; Yener FG; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul 34116, Turkey.
- Source
- Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
- Subject
- Language
- English
Biomimetic three-layered monolithic scaffold (TLS) intended for treatment of osteochondral defects was fabricated by using freeze drying method. The multilayered material was prepared with chitosan (C) and alginate (A) polyelectrolyte complex (CA/PEC) as a cartilaginous layer, a combination of CA/PEC (60 wt%) and β-tricalcium phosphate (β-TCP) (40 wt%) as an intermediate layer and a combination of CA/PEC (30 wt%) and β-TCP (70 wt%) as a subchondral layer in order to mimic the inherent gradient structure of healthy osteochondral tissue. Characterization of the scaffolds was performed using Fourier transform infrared (FT-IR) spectroscopy analysis, swelling and scanning electron microscopy (SEM) tests. In vitro cytotoxicity assay with L929 cells and EpiDerm skin irritation test (SIT) using the EpiDerm reconstructed human epidermal (RHE) model were performed to analyze biocompatibility of the scaffolds. Characterization results showed that there were strong ionic interactions among chitosan, alginate and β-TCP and the layers showed interconnected porous structure with different swelling ratios. The relative cell viability and SIT results were greater than 70% indicating that the scaffolds are considered nontoxic according to the International Organization for Standardization (ISO) standard. All results taken together, biomimetic TLS can be considered to be suitable for osteochondral applications.
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