Synthesis, characterization, pharmacological and computational evaluation of hyaluronic acid modified chebulinic acid encapsulated chitosan nanocomposite for cancer therapy.
- Resource Type
- Academic Journal
- Authors
- Shah HS; Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan. Electronic address: hamid.saeed@uvas.edu.pk.; Zaib S; Department of Basic and Applied Chemistry, Faculty of Science and Technology, University of Central Punjab, Lahore 54590, Pakistan. Electronic address: sumera.zaib@ucp.edu.pk.; Usman F; Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 66000, Pakistan. Electronic address: faisal.usman@bzu.edu.pk.; Sarfraz M; College of Pharmacy, Al Ain University, Al Ain 64141, United Arab Emirates. Electronic address: muhammad.sarfraz@aau.ac.ae.; Faiz R; Department of Zoology, University of Education, Bank Road Campus, Lahore, Pakistan. Electronic address: rabia.faiz@ue.edu.pk.; Rehman SA; Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan; M Islam College of Pharmacy, 52230 Gujranwala, Pakistan.; Khan AA; Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: azkhan@ksu.edu.sa.; Alanazi AM; Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.; Khan R; College of Pharmacy, University of Sargodha, 40100 Sargodha, Pakistan.; Nasrullah U; Institute of General Pharmacology and Toxicology, University Hospital Frankfurt, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany. Electronic address: nasrullah@em.uni-frankfurt.de.; Nazir I; Department of Pharmacy, COMSATS University Islamabad, Lahore campus, 54000 Lahore, Pakistan. Electronic address: imrannazir@cuilahore.edu.pk.
- Source
- Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
- Subject
- Language
- English
The purpose of this study was to produce hyaluronic acid customized nanoparticles with chitosan for the delivery of chebulinic acid (CLA) to enhance its anticancer potential against breast cancer. A significant portion of CLA was encapsulated (89.72 ± 4.38 %) and loaded (43.15 ± 5.61 %) within hybrid nanoparticles. The colloidal hybrid nanoparticles demonstrated a polydispersity index (PDI) of about 0.379 ± 0.112, with zeta capacitance of 32.69 ± 5.12 (mV), and an average size of 115 ± 8 (nm). It was found that CLA-CT-HA-NPs had stronger anticancer effects on MCF-7 cells (IC 50 = 8.18 ± 3.02 μM) than pure CLA (IC 50 = 17.15 ± 5.11 μM). The initial cytotoxicity findings were supported by additional investigations based on comet assay and flow cytometry analysis. Tumor remission and survival were evaluated in five separate groups of mice. When juxtaposed with pure CLA (3.17 ± 0.419 %), CLA-CT-HA-NPs improved survival rates and reduced tumor burden by 3.76 ± 0.811(%). Furthermore, in-silico molecular docking investigations revealed that various biodegradable polymers had several levels of compatibility with CLA. The outcomes of this study might potentially served as an effective strategy for delivering drugs in the context of breast cancer therapy.
Competing Interests: Declaration of competing interest The authors state that there are no competing interests involved.
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