Rapid detection of Tobacco streak virus (TSV) in cotton (Gossypium hirsutum) based on Reverse Transcription Loop Mediated Isothermal Amplification (RT-LAMP).
- Resource Type
- Academic Journal
- Authors
- Gawande SP; Division of Crop Protection, ICAR-Central Institute for Cotton Research (CICR), Nagpur, 440010, India. Electronic address: spgawande1@gmail.com.; K P R; Division of Crop Improvement, ICAR-Central Institute for Cotton Research (CICR), Nagpur, 440010, India.; Monga D; ICAR- Central Institute for Cotton Research (CICR), Regional Station, Sirsa, 125055, India.; Nagrale DT; Division of Crop Protection, ICAR-Central Institute for Cotton Research (CICR), Nagpur, 440010, India.; Kranthi S; Division of Crop Protection, ICAR-Central Institute for Cotton Research (CICR), Nagpur, 440010, India.
- Source
- Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
- Subject
- Language
- English
Tobacco Streak Virus (TSV) belongs to the genus Ilarvirus of the family Bromoviridae an emerging pathogen posing threat to the crop species worldwide. Identification of symptoms due to TSV infection by visual observation of plants often results in misdiagnosis as symptoms produced by this virus can match with those reflecting physiological and nutritional disorders affecting cotton. Development of diagnostic tools with rapidity will have immense role to play in detection and management of the emerging virus. The protocol for rapid diagnosis of TSV infected samples by using Reverse Transcription-Loop Mediated Isothermal Amplification (RT-LAMP) was optimised and this is the first report of its use for diagnosis of TSV on cotton and Soybean. The colorimetric detection for diagnostic simplicity of amplified RT-LAMP product by using different dyes lead to enhanced applicability of this technique. The RT-LAMP diagnostic tool can be utilized not only for laboratory research but also for quarantine and field diagnosis of this important emerging pathogen affecting cotton.
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