Next-generation sequencing using tissue specimen collected with a 1.1 mm-diameter cryoprobe in patients with lung cancer.
- Resource Type
- Academic Journal
- Authors
- Kim MH; Department of Internal Medicine, Pusan National University School of Medicine, Busan, Republic of Korea.; Department of Internal Medicine, Pusan National University Hospital, Busan, Republic of Korea.; Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.; Kim SH; Department of Internal Medicine, Pusan National University School of Medicine, Busan, Republic of Korea.; Department of Internal Medicine, Pusan National University Hospital, Busan, Republic of Korea.; Lee G; Department of Radiology, Pusan National University School of Medicine, Busan, Republic of Korea.; Department of Radiology, Pusan National University Hospital, Busan, Republic of Korea.; Mok J; Department of Internal Medicine, Pusan National University School of Medicine, Busan, Republic of Korea.; Department of Internal Medicine, Pusan National University Hospital, Busan, Republic of Korea.; Lee MK; Department of Internal Medicine, Pusan National University School of Medicine, Busan, Republic of Korea.; Department of Internal Medicine, Pusan National University Hospital, Busan, Republic of Korea.; Song JS; GC Genome Corporation, Yongin, Republic of Korea.; Eom JS; Department of Internal Medicine, Pusan National University School of Medicine, Busan, Republic of Korea.; Department of Internal Medicine, Pusan National University Hospital, Busan, Republic of Korea.; Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
- Source
- Publisher: Blackwell Science Country of Publication: Australia NLM ID: 9616368 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1440-1843 (Electronic) Linking ISSN: 13237799 NLM ISO Abbreviation: Respirology Subsets: MEDLINE
- Subject
- Language
- English
Background and Objective: Next-generation sequencing (NGS) analysis is considered standard for lung cancer diagnosis in clinical practice. Little is known about the feasibility of NGS using tumour tissue sampled with a 1.1 mm-diameter cryoprobe. We aimed to investigate the suitability of specimens obtained by transbronchial cryobiopsy (TBC) using a 1.1 mm-diameter cryoprobe for NGS analysis.
Methods: Patients with lung cancer who underwent TBC using a 1.1 mm-diameter cryoprobe for NGS testing between October 2020 and April 2023 were enrolled. A 4.0- or 3.0 mm-diameter bronchoscope with radial probe endobronchial ultrasound and virtual bronchoscopic navigation was used to detect peripheral lung lesions. All procedures were performed under fluoroscopic guidance. Data were analysed retrospectively.
Results: A total of 56 patients underwent TBC using a 1.1 mm cryoprobe for NGS testing, during the study period. Most patients (98%) were in the advanced stage of lung cancer (recurrent or inoperable disease of stages III or IV). The diagnostic yield of NGS for DNA and RNA sequencing was 95% each (53 of 56). Moderate bleeding was noted in three patients (5%) and none of the study patients developed life-threatening complications, such as pneumothorax or lung infection.
Conclusion: TBC using a 1.1 mm-diameter cryoprobe is a useful and safe tool for NGS analysis, for both DNA and RNA sequencing.
(© 2024 Asian Pacific Society of Respirology.)