Genetic encoding of isobutyryl-, isovaleryl-, and β-hydroxybutryl-lysine in E. coli .
- Resource Type
- Academic Journal
- Authors
- Christopher JA; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu.; Galbada Liyanage SA; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu.; Nicholson EM; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu.; Kinney WD; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu.; Cropp TA; Department of Chemistry, Virginia Commonwealth University Richmond VA 23284 USA tacropp@vcu.edu.
- Source
- Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101581657 Publication Model: eCollection Cited Medium: Internet ISSN: 2046-2069 (Electronic) Linking ISSN: 20462069 NLM ISO Abbreviation: RSC Adv Subsets: PubMed not MEDLINE
- Subject
- Language
- English
Here we report the synthesis and genetic encoding of the lysine post translational modifications, β-hydroxybutyryl-lysine, isobutyryl-lysine and isovaleryl-lysine. The ability to obtain a homogenous protein samples with site-specific incorporation of these acylated lysine residues can serve as a powerful tool to study the biological role of lysine post translational modifications.
Competing Interests: The authors indicate there are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)