Identification and characterisation of functional K ir 6.1-containing ATP-sensitive potassium channels in the cardiac ventricular sarcolemmal membrane.
- Resource Type
- Academic Journal
- Authors
- Brennan S; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.; Chen S; Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.; Makwana S; Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.; Esposito S; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.; Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.; McGuinness LR; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.; Alnaimi AIM; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.; Department of Cardiac Technology, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.; Sims MW; Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.; Patel M; Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.; Aziz Q; William Harvey Research Institute, Queen Mary University of London, London, UK.; Ojake L; William Harvey Research Institute, Queen Mary University of London, London, UK.; Roberts JA; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.; Sharma P; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.; Lodwick D; Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.; Tinker A; William Harvey Research Institute, Queen Mary University of London, London, UK.; Barrett-Jolley R; Department of Musculoskeletal and Ageing Science, University of Liverpool, Liverpool, UK.; Dart C; Department of Biochemistry, Cell and Systems Biology, University of Liverpool, Liverpool, UK.; Rainbow RD; Department of Cardiovascular and Metabolic Medicine and Liverpool Centre for Cardiovascular Science, University of Liverpool, Liverpool, UK.
- Source
- Publisher: Wiley Country of Publication: England NLM ID: 7502536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5381 (Electronic) Linking ISSN: 00071188 NLM ISO Abbreviation: Br J Pharmacol Subsets: MEDLINE
- Subject
- Language
- English
Background and Purpose: The canonical K ir 6.2/SUR2A ventricular K ATP channel is highly ATP-sensitive and remains closed under normal physiological conditions. These channels activate only when prolonged metabolic compromise causes significant ATP depletion and then shortens the action potential to reduce contractile activity. Pharmacological activation of K ATP channels is cardioprotective, but physiologically, it is difficult to understand how these channels protect the heart if they only open under extreme metabolic stress. The presence of a second K ATP channel population could help explain this. Here, we characterise the biophysical and pharmacological behaviours of a constitutively active K ir 6.1-containing K ATP channel in ventricular cardiomyocytes.
Experimental Approach: Patch-clamp recordings from rat ventricular myocytes in combination with well-defined pharmacological modulators was used to characterise these newly identified K + channels. Action potential recording, calcium (Fluo-4) fluorescence measurements and video edge detection of contractile function were used to assess functional consequences of channel modulation.
Key Results: Our data show a ventricular K + conductance whose biophysical characteristics and response to pharmacological modulation were consistent with K ir 6.1-containing channels. These K ir 6.1-containing channels lack the ATP-sensitivity of the canonical channels and are constitutively active.
Conclusion and Implications: We conclude there are two functionally distinct populations of ventricular K ATP channels: constitutively active K ir 6.1-containing channels that play an important role in fine-tuning the action potential and K ir 6.2/SUR2A channels that activate with prolonged ischaemia to impart late-stage protection against catastrophic ATP depletion. Further research is required to determine whether K ir 6.1 is an overlooked target in Comprehensive in vitro Proarrhythmia Assay (CiPA) cardiac safety screens.
(© 2024 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society.)