Summary: Lastly, we developed dynamically controlled micropatterned wavy substrates for on-demand reversible cell alignment. PDMS substrates were plasma treated and compressed to obtain reversible wavy microfeatures on the surface, and were capable of aligning cells into a highly ordered configuration from random orientation. Muscle cells cultured on these surfaces initially attached in a random order, aligned once the substrate was compressed, and unaligned when the substrate was released. This technique provides a simple and reliable method by which to investigate developmental processes not yet easily studied in vitro.