Purification and Characterization of Aromatic Amine Dehydrogenase from Alcaligenes xylosoxidans.
- Resource Type
- Article
- Authors
- Kondo, Tetsuya; Kondo, Emi; Maki, Hitomi; Yasumoto, Kyoden; Takagi, Kazuyoshi; Kano, Kenji; Ikeda, Tokuji
- Source
- Bioscience, Biotechnology & Biochemistry. Sep2004, Vol. 68 Issue 9, p1921-1928. 8p. 3 Charts, 7 Graphs.
- Subject
- *AMINES
*DEHYDROGENASES
*ALCALIGENES
*GEL electrophoresis
*AMINO acids
*NUCLEOTIDE sequence
- Language
- ISSN
- 0916-8451
Reports on the purification and characterization of the aromatic amine dehydrogenase from Alcaligenes xylosoxidans IFO13495 grown on beta-phenylethylamine. Microorganism and cultivation; Determination of the aromatic amine dehydrogenase activity; Polyacrylamide gel electrophoresis in the absence of sodium dodecylsulfate; Measurement of molecular mass; N-terminal amino acid sequencing.