In this work, amino functionalized lotus seedpods-derived biochar (LSBC-NH2) was prepared for the removal of uranium (VI) from aqueous solution by using natural lotus seedpods as raw material and urea as nitrogen source through hydrothermal method. LSBC-NH2 showed a very thin sheet structure with rough porous surface and cluster structure and good thermal stability. Kinetics, isotherms and thermodynamics studies demonstrated that the adsorption of uranium (VI) by LSBC-NH2 was a spontaneous, endothermic, and single-layer chemisorption process, and the maximum uranium (VI) adsorption capacity on LSBC-NH2 could reach 367.99 mg g−1. Adsorption mechanism showed that the adsorption process of LSBC-NH2 for uranium (VI)was involved in amino, carboxyl and hydroxyl groups in LSBC-NH2. [ABSTRACT FROM AUTHOR]